Tracking variations in wetland use by breeding flamingos using stable isotope signatures of feather and blood

This study tracks temporal variations in the habitat use of breeding adult greater flamingos (Phoeni copterus roseus) in the Camargue (southern France) using simultaneous sampling of dN, dC and dS from feather and blood of chicks at fledging. Camargue flamingos forage in a heterogeneous complex of saltpans, permanent and temporary brackish lagoons, freshwater and euryhaline marshes to provision their chicks over a two month period. Using Bayesian mixing models with diet and tissue specific discrimination factors and invertebrates collected from 23 locations, we investigated whether blood and feather isotopes indicated temporal variations in habitat use relative to salinity and hydroperiod. We also tested whether fledgling body condition could be explained by the isotopic signatures of their tis sues. While dC and dN values did not differ significantly between blood and feather, marked differ ences were apparent in the dS values obtained from these tissues. Saltpans (38%) and freshwater marshes (33%) appeared to be the main habitats visited by adult birds in the early phase of parental care with use of saline wetlands increasing later in the season (54%). This habitat shift may be related to the peak of resources in saltpans and the drying up of freshwater and brackish marshes by mid summer. Habitat shift (as expressed in individual shifts in isotope values between feather and blood) was not correlated with offspring age, and differed between individuals. A negative relationship was observed between offspring body condition and feather dN, indicating that chicks fed from temporary flooded marshes fare better than those provisioned from permanent marshes. Foraging strategies of greater flamingos during parental care were heterogeneous, possibly tracking changes in resource availability as the season progressed and reflecting differences in the competitive ability of parent birds. Given that the Camargue saltpans face closure and the region’s temporary wetlands remain threatened, our results emphasize the critical importance of conserving these two key habitats for greater flamingos. Dual tissue, triple stable isotope analysis provides a useful and sensitive means of tracking localized environmental change in this threatened system.